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Fig. 1 | Plant Methods

Fig. 1

From: Rapid phenotyping of crop root systems in undisturbed field soils using X-ray computed tomography

Fig. 1

CT results. 3D RSAs of barley seedlings (a, b) grown for 14 days in a 6 cm diameter pot filled with Haplic luvisol (Campus Klein-Altendorf) and grass–legume mixture (c) grown for 24 months in an Pseudogleyed Cambisol (Agroscope research station Reckenholz), sampled in undisturbed fashion in situ using a 3.4 cm diameter cylinder. In a roots were segmented by a region-growing algorithm (80 min active working time), and in b, c the roots were segmented using the presented protocol (5 min active working time). Root diameter distribution of c is shown in color-coded scale (+1 min of active working time for diameter measurement = 6 min). The distribution of the frequency of root surface elements with a specific root diameter was calculated (d). Root dry mass density is plotted against root volume as reconstructed by CT (e); **significant, linear correlation on P-level 0.01 (n = 18)

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