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Fig. 3 | Plant Methods

Fig. 3

From: A high-throughput pipeline for detecting locus-specific polymorphism in hexaploid wheat (Triticum aestivum L.)

Fig. 3

Validation of marker locations using a DH (doubled haploid) population. Orthologous sequences of Bradi1g54730.1 and Bradi1g55847.1 were amplified from the two parents of the DH population, B (‘Batavia’) and E (‘Ernie’), and sequenced. The single nucleotide polymorphism (in red and green) and restriction enzyme sites (underlined) were identified between B and E for Bradi1g54730.1 with restriction enzyme AciI (a) and Bradi1g55847.1 with HaeIII (b). The amplified products of the two parents and 14 of the DH lines were digested and separated on agarose gels. Map positions of the two new markers on chromosome 2B (c) were calculated based on the linkage map published by Li et al. [21].

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Plant Methods

ISSN: 1746-4811