Skip to main content
Figure 6 | Plant Methods

Figure 6

From: A pin-fasten grafting method provides a non-sterile and highly efficient method for grafting Arabidopsis at diverse developmental stages

Figure 6

Development of symplastic connection between stocks and scions. Arabidopsis gl1-1 scions were grafted onto wild-type stocks under LD conditions. The 5(6)-carboxyfluorescein diacetate dye (green fluorescence) was introduced onto leaves (indicated by arrows) of wild-type stocks at different times after grafting. The images (left panels) and florescence images (right panels) were taken at 4 days (a, b), 5 days (c, d), 6 days (e, f), and 8 days (g, h) after grafting. Note green fluorescent signals (or yellow colors when merged with red florescence) in the vasculature of gl1-1 scions at 6 or 8 days after grafting but not 4 or 5 days after grafting. The red colors in florescence images represents the auto-florescence of chlorophyll. The glabrous leaves of scions are indicated by white asterisks (left panels).

Back to article page

Plant Methods

ISSN: 1746-4811