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Figure 1 | Plant Methods

Figure 1

From: A pin-fasten grafting method provides a non-sterile and highly efficient method for grafting Arabidopsis at diverse developmental stages

Figure 1

Arabidopsis pin-fasten grafting under long-day (LD) growth conditions. a Arabidopsis pin-fasten grafting. Ten- to 12-day-old Arabidopsis seedlings were decapitated from apical meristems (stocks green) and pin-fastened with scions (blue). The insect pin is indicated by a red line. b, c Images of 10- to 12-day-old LD-grown Arabidopsis seedlings used as stocks (b) or scions (c). d Apex-removed stock. The removed apex is shown in the inset. Scale is 1 mm. Note that only the apex and emerged young leaf primodia were removed. e Scions with an insect pin inserted from the base of the petiole through the hypocotyl. f Magnified images of apex-removed stocks to show the diameter of a cut area. Scale bar is 1 mm. g, h Grafted plants with a gl1-1 scion assembled on a wild-type stock. i Image of successful grafting plants at 14 days after grafting. The 6th leaf of the stocks is indicated by a white arrow in (b, d, f). Red dashed lines in (g) represent the stems of scions (upper) and stocks (lower). White asterisks in (h, i) indicate the original and newly developed leaves of gl1-1 scions.

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