Skip to main content

Table 1 Antibiotic selection conditions for C. richardii transformation

From: Protocol: genetic transformation of the fern Ceratopteris richardii through microparticle bombardment

Antibiotic Concentration (μg/ml) required for screening
Callus Gametophyte Sporophyte
Hygromycin B 40 20 20
G-418 50 20 20
  1. Final concentrations of antibiotic (as specified) required in growth media for successful identification of transgenic tissues from callus, gametophyte and sporophyte populations in tissue culture. Hygromycin B and G-418 concentrations were determined empirically ([9]; Additional file 2).