Biases underlying species detection using fluorescent amplified-fragment length polymorphisms yielded from roots

Table 2 Detection success of amplicons yielded from multi-species mixtures

cpDNA	Fragment size (bp)	Species	Detection rate
cpDNA	Fragment size (bp)	Species	2.73 ng μL⁻¹ DNA	12.5 ng μL⁻¹ DNA	22.73 ng μL⁻¹ DNA
trnT-trnL intergenic spacer	377	Bromus inermis ^a	0/3	3/3	3/3
trnT-trnL intergenic spacer	466	Picea glauca ^a	0/3	0/3	0/3
trnT-trnL intergenic spacer	475	Picea glauca ^a	0/3	0/3	0/3
trnT-trnL intergenic spacer	530	Populus tremuloides	3/3	3/3	3/3
trnT-trnL intergenic spacer	664	Bromus inermis ^a	3/3	3/3	3/3
trnT-trnL intergenic spacer	1,154	Melilotus officinalis	0/3	0/3	0/3

cpDNA	Fragment size (bp)	Species	Detection rate
cpDNA	Fragment size (bp)	Species	0.045 ng μL⁻¹ DNA	0.25 ng μL⁻¹ DNA	0.45 ng μL⁻¹ DNA
trnL intron	319	Melilotus officinalis	3/3	3/3	3/3
trnL intron	508	Sonchus arvensis	3/3	3/3	3/3
trnL intron	603	Chamerion angustifolium	0/3	0/3	0/3
trnL intron	706	Populus tremuloides	0/3	0/3	0/3
trnL-trnF intergenic spacer	209	Trifolium hybridum	3/3	3/3	3/3
trnL-trnF intergenic spacer	365	Chamerion angustifolium	3/3	3/3	3/3
trnL-trnF intergenic spacer	394	Bromus inermis ^a	0/3	0/3	0/3
trnL-trnF intergenic spacer	443	Bromus inermis ^a	0/3	3/3	3/3
trnL-trnF intergenic spacer	476	Populus tremuloides	0/3	3/3	3/3

DNA was extracted from leaves of identified species and mixed in varied proportions with other known plant species. Numerator is number of successful detections; denominator is number of trials.
^aThis species yielded fragments of different sizes.

ISSN: 1746-4811