Skip to main content

Table 2 Detection success of amplicons yielded from multi-species mixtures

From: Biases underlying species detection using fluorescent amplified-fragment length polymorphisms yielded from roots

cpDNA Fragment size (bp) Species Detection rate
2.73 ng μL−1 DNA 12.5 ng μL−1 DNA 22.73 ng μL−1 DNA
trnT-trnL intergenic spacer 377 Bromus inermis a 0/3 3/3 3/3
trnT-trnL intergenic spacer 466 Picea glauca a 0/3 0/3 0/3
trnT-trnL intergenic spacer 475 Picea glauca a 0/3 0/3 0/3
trnT-trnL intergenic spacer 530 Populus tremuloides 3/3 3/3 3/3
trnT-trnL intergenic spacer 664 Bromus inermis a 3/3 3/3 3/3
trnT-trnL intergenic spacer 1,154 Melilotus officinalis 0/3 0/3 0/3
cpDNA Fragment size (bp) Species Detection rate
0.045 ng μL−1 DNA 0.25 ng μL−1 DNA 0.45 ng μL−1 DNA
trnL intron 319 Melilotus officinalis 3/3 3/3 3/3
trnL intron 508 Sonchus arvensis 3/3 3/3 3/3
trnL intron 603 Chamerion angustifolium 0/3 0/3 0/3
trnL intron 706 Populus tremuloides 0/3 0/3 0/3
trnL-trnF intergenic spacer 209 Trifolium hybridum 3/3 3/3 3/3
trnL-trnF intergenic spacer 365 Chamerion angustifolium 3/3 3/3 3/3
trnL-trnF intergenic spacer 394 Bromus inermis a 0/3 0/3 0/3
trnL-trnF intergenic spacer 443 Bromus inermis a 0/3 3/3 3/3
trnL-trnF intergenic spacer 476 Populus tremuloides 0/3 3/3 3/3
  1. DNA was extracted from leaves of identified species and mixed in varied proportions with other known plant species. Numerator is number of successful detections; denominator is number of trials.
  2. aThis species yielded fragments of different sizes.