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Figure 3 | Plant Methods

Figure 3

From: Biases underlying species detection using fluorescent amplified-fragment length polymorphisms yielded from roots

Figure 3

Relationship between DNA template concentration, extraction efficiency and detection thresholds. Shown is the extraction efficiency of a target species compared with matrix species, and the detectable percentage of sample comprising the target species across a range of the minimum DNA concentration required for amplification: a 0.05, b 0.075 and c 0.09 ng μL−1. Let Ds be the percentage of root material of the target species in the mixture, then the portion of the target species in the DNA extract, Ps = Ds × Es/(Ds × Es + (100 − Ds) × Em), where Es is the DNA extraction efficiency of the target species, in ng μL−1 mg−1, and Em is the DNA extraction efficiency of the other species in the matrix, in ng μL−1 mg−1. If Csmin is the minimum DNA concentration of the target species required for successful amplification of the focal fragment, in ng μL−1, then the minimum concentration of the DNA template required for amplification, C in ng μL−1, is given by C = Csmin/Ps, where C = Csmin × (Ds × Es + (100 − Ds) × Em)/(Ds × Es) or C = Csmin × (1 + ((100 − Ds)/Ds) × (Em/Es)).

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