Hyperspectral imaging microscope setup for small-scale image analysis. The spectral reflectance of detached barley leaves on phyto agar was measured with a hyperspectral camera, which was mounted on a magnification lens to enable a magnification up to 7.3x. Two linear light emitters with a vertical orientation of 30° illuminated the samples in a distance of 20 cm. The samples were moveable due to a XY moving stage. A spectral resolution of up to 2.73 nm and a maximum spatial resolution of 7.5 μm per pixel was obtained. The field of view ranged from 4.1 to 0.95 cm, dependent on the magnification.