Scheme of the grow chamber-based automated high-throughput phenotyping platform PlantScreen™ (Photons Systems Instruments, Brno, Czech Republic), installed at Palacký University in Olomouc, Czech Republic . The system is located in a growth chamber with white LED illumination (max. 1000 μmol photons m−2 s−1) and controlled environment (10 – 40°C, 30 – 99% relative humidity). The growth area with roller conveyer has capacity of up to 640 Arabidopsis, cereals and other crops grown in standardized pots. The measuring cabinet contains acclimation chamber for dark adaptation of plants coupled with an automated weighting and watering area. The cabinet is equipped with KCFIM and RGB imaging (top and 2 side views), thermoimaging (IR) to measure stomata openness and SWIR hyperspectral imaging to determine water content. The platform can be controlled either from the place or via remote control software. The operating software enables automatic data evaluation.