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Table 1 Summary comparison of circadian screening techniques

From: A comparison of high-throughput techniques for assaying circadian rhythms in plants

Assay

Type

Species suitability

Requires

Max resolution

Throughput

Ref.

   

transformation?

   

Western Blot/qPCR

Direct assay of

All plants

No

Single Cell/Tissue

Low

[89]

 

gene expression.

     

Luciferase

Direct assay of

Transformable plants

Yes

Single Cell/Tissue

High

[36]

 

gene transcription.

     

Delayed fluorescence

Indirect assay of

Most plants

No

Whole plant

High

[58]

 

clock phenotype.

     

Leaf movement

Indirect assay of

Most Dicots

No

Whole plant

High

[67,69]

 

clock phenotype.

     

IRGA

Indirect assay of

All plants

No

Whole organ (Individual leaves)

Medium

[73]

 

clock phenotype

    Â