High-throughput analysis of Arabidopsis growth and chlorophyll fluorescence in the PHENOPSIS automaton. (A)
A. thaliana plants are grown in controlled environmental conditions in the PHENOPSIS platform equipped with a chlorophyll fluorescence imaging system. (B) Dynamics of soil relative water content in two watering scenarios including constant well-watered conditions (WW) and water withdrawing from the four-leaves stage (L4; beginning of stress) followed by rewatering after 1 day at 6% g H2O−1 dry soil (SWD). Data are means (± SE) of 13 and 48 plants under WW and SWD, respectively. (C) Plant growth (top) and whole-rosette F
m (bottom) during plant development and under SWD. F
m values are represented by false colour scale ranging from black (pixel values 0) through red, yellow, green, blue to purple (ending at 1). (D) Visible images of surviving and perishing plants (left) and survival percentage of plants under WW and SWD conditions (right). Asterisks indicate significant differences following Chi2 test between plants grown in WW conditions (n = 13) and plants under SWD (n = 19 and 29 for surviving and perishing plants, respectively; ***: P < 0.001). (E) Total projected leaf area of plants under WW conditions and SWD (surviving and perishing plants) as a function of days after L4 stage until bolting. Data are means (± SE) of 13–29 plants.