Table 4 De novo assembly and annotation of C. japonica contigs classified in two different approaches

Library type

Illumina, 74 bp single-end

Illumina, 74 bp single-end

Assembler

Velvet/Oases

Velvet/Oases

Assembled reads

141,089,611

205,957,611

Number of contigs

59,449

249,621

Median contig length

325 bp

187 bp

Average contig length

648 bp

354 bp

Length distribution (%)

<200 bp

18,009 (30.3)

134,968 (54.1)

201-500 bp

19,803 (33.3)

75,199 (30.1)

501-1000 bp

9,739 (16.4)

22,305 (8.9)

>1001 bp

11,898 (20.0)

17,149 (6.9)

Length distribution of contigs no-hits-found in refseqplant (%)

<200 bp

13,986 (23.5)

114,864 (46.0)

201-500 bp

12,232 (20.6)

53,096 (21.3)

501-1000 bp

3,160 (5.3)

10,616 (4.3)

>1001 bp

952 (1.6)

2,639 (1.1)