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Figure 6 | Plant Methods

Figure 6

From: An optical imaging chamber for viewing living plant cells and tissues at high resolution for extended periods

Figure 6

The addition and removal of PI in a growing Arabidopsis root during image acquisition. The root was imaged by confocal microscopy where z stacks were taken every 5 minutes. (a) Only green fluorescent nuclei (GFP-eIF4A-III) are visible at the start of the experiment (b) Within 5 minutes after the addition of PI cell outlines become clearly visible (c) PI was washed out of the chamber leaving only a faint red outline. Images are summation projection of the z stacks. Scale bar = 20 μm.

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Plant Methods

ISSN: 1746-4811