Figure 5

Increasing the field of view using tiled imaging to capture the growth of an Arabidopsis leaf expressing GFP tubulin. (a) The dashed box indicates the limited field of view from 40/1.2 water objective at a single position. Tiled images were captured every hour for 63 hours where cell division (arrow) and growth were captured. Scale bar = 100 μm. (b) Displays a sub region from the tiled image (panel (a) highlighted in green) illustrating cellular growth. Maximum projections of the z stacks are shown and the numbers indicate time in hours from start of time-lapse experiment. Scale bar = 30 μm.