Figure 4

High resolution imaging of a dividing Arabidopsis suspension cell. Confocal microscopy time-lapse images of an Arabidopsis culture cell expressing GFP tubulin. High resolution z stack images were collected using a 63/1.4 oil immersion objective, every 5 minutes throughout division. This illustrates the interchange of different microtubule structures: prometaphase (0 min); metaphase (5 min); anaphase (10 min); telophase (15 min daughter nuclei position indicated by arrows); phragmoplast (20 min); cell division completion (85 min). The numbers indicate the time in minutes from start of time-lapse acquisition and confocal z stacks are shown as summation projections. Scale bar = 10 μm.