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Table 1 Plasmids used to transform C. reinhardtii

From: TETX: a novel nuclear selection marker for Chlamydomonas reinhardtii transformation

Plasmid

Promoter/terminator/#RBCS2 introns

Resistance

Base pairs/MW (g/mol)

TE

Source

AtetX

HSP70A:RBCS2/RBCS2/1

Tetracycline

5238/3236640.1

6.18

This work

BtetX

β-2 tubulin/COP-1/NO

Tetracycline

4445/2746642.9

3.28

This work

pKs-aphVIII

β-2 tubulin/COP-1/NO

Paromomycin

4308/2661961.7

4.51

[38]

psP124S-ble

RBCS2/RBCS2/2

Zeocin

4133/2553770

22.56

[5]

  1. Listing of plasmids used in this work with their corresponding promoter/terminator/number of RBCS2 introns, associated resistance, plasmid size, molecular weight (MW) and Transformation Efficiency (TE).
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Plant Methods

ISSN: 1746-4811