Figure 2
From: Ion Torrent sequencing as a tool for mutation discovery in the flax (Linum usitatissimum L.) genome
Sequence coverage and frequency of variants in gene sections of the pilot experiment. DNA from the cultivar Macbeth was diluted (1:64 or 1:96) in CDC Bethune DNA in several simulated pools as described in methods. Each line in the sequence coverage graphs represents one of 28 pools. The frequencies of the variants are plotted against the position in the respective reference sequence section. Each symbol in the frequency graphs represents a frequency of at least 0.1% for each of the 28 pools in each position. A. S20, B. S900; no graphs for S411 are shown since no variants were detected in that sequence.