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Table 1 Identification and chromatographic data of carotenoid standards

From: A rapid and sensitive method for determination of carotenoids in plant tissues by high performance liquid chromatography

Peak no.

Compound

RT (min)

λ (nm) found

%III/II found

Regression equation

R 2

LOD

LOQ

k

α

%CV

1

All-trans-violaxanthin

4.05

416.0, 439.0, 469.0

88.8

y = 1.011x - 0.270

0.991

0.075

0.250

1.66

1.10

5.45

2

All-trans-neoxanthin

4.31

415.0, 437.0, 465.0

78.2

y = 1.042x −1.842

0.996

0.075

0.250

1.83

1.17

5.30

3

All-trans-antheraxanthin

4.80

(424.0), 446.0, 474.0

60.0

y = 1.057x −1.919

0.994

0.075

0.250

2.15

1.13

4.94

4

All-trans lutein

5.25

(424.0), 445.0, 474.0

62.5

y = 1.000x - 0.040

0.997

0.075

0.250

2.45

1.12

4.43

5

All-trans-zeaxanthin

5.73

(428.0), 451.0, 478.0

40.0

y = 1.046x - 1.725

0.998

0.075

0.250

2.76

1.37

5.66

6

15-cis-phytoene

7.29

(277.0), 286.0, (298.0)

n.c.

y = 1.066x - 1.081

0.989

0.120

0.400

3.79

1.06

7.74

7

All-trans- β-cryptoxanthin

7.66

(424.0), 452.0, 479.0

35.0

y = 1.028x - 1.282

0.991

0.075

0.250

4.03

1.12

6.84

8

All-trans-phytofluene

8.43

332.0, 348.0, 367.0

90.9

y = 1.001x - 0.118

0.998

0.120

0.400

4.54

1.06

6.60

9

All-trans- α-carotene

8.84

424.0, 446.0, 475.0

63.3

y = 1.012x - 0.892

0.991

0.075

0.250

4.81

1.07

7.94

10

All-trans- β-carotene

9.42

(425.0), 452.0, 479.0

30.0

y = 1.016x - 1.176

0.990

0.075

0.250

5.19

1.09

6.36

11

All-trans- ζ-carotene

10.18

380.0, 403.0, 426.0

115.4

y = 1.015x - 1.092

0.991

0.075

0.250

5.69

1.14

7.69

12

All-trans- δ-carotene

11.43

432.0, 457.0, 488.0

71.4

y = 1.014x - 1.015

0.989

0.075

0.250

6.51

1.06

5.22

13

All-trans- γ-carotene

12.09

439.0, 462.0, 492.0

53.3

y = 1.008x - 0.396

0.983

0.075

0.250

6.95

1.02

8.40

14

All-trans-neurosporene

12.40

416.0, 440.0, 469.0

92.3

y = 1.010x - 1.041

0.991

0.075

0.250

7.15

1.25

4.86

15

All-trans-lycopene

15.09

446.0, 472.0, 503.0

73.9

y = 1.027x - 0.630

0.993

0.075

0.250

8.92

1.37

10.67

  1. Parentheses indicate a shoulder.
  2. %III/II represents the ratio of peak heights from the trough between peak II and III.