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Table 1 Identification and chromatographic data of carotenoid standards

From: A rapid and sensitive method for determination of carotenoids in plant tissues by high performance liquid chromatography

Peak no. Compound RT (min) λ (nm) found %III/II found Regression equation R 2 LOD LOQ k α %CV
1 All-trans-violaxanthin 4.05 416.0, 439.0, 469.0 88.8 y = 1.011x - 0.270 0.991 0.075 0.250 1.66 1.10 5.45
2 All-trans-neoxanthin 4.31 415.0, 437.0, 465.0 78.2 y = 1.042x −1.842 0.996 0.075 0.250 1.83 1.17 5.30
3 All-trans-antheraxanthin 4.80 (424.0), 446.0, 474.0 60.0 y = 1.057x −1.919 0.994 0.075 0.250 2.15 1.13 4.94
4 All-trans lutein 5.25 (424.0), 445.0, 474.0 62.5 y = 1.000x - 0.040 0.997 0.075 0.250 2.45 1.12 4.43
5 All-trans-zeaxanthin 5.73 (428.0), 451.0, 478.0 40.0 y = 1.046x - 1.725 0.998 0.075 0.250 2.76 1.37 5.66
6 15-cis-phytoene 7.29 (277.0), 286.0, (298.0) n.c. y = 1.066x - 1.081 0.989 0.120 0.400 3.79 1.06 7.74
7 All-trans- β-cryptoxanthin 7.66 (424.0), 452.0, 479.0 35.0 y = 1.028x - 1.282 0.991 0.075 0.250 4.03 1.12 6.84
8 All-trans-phytofluene 8.43 332.0, 348.0, 367.0 90.9 y = 1.001x - 0.118 0.998 0.120 0.400 4.54 1.06 6.60
9 All-trans- α-carotene 8.84 424.0, 446.0, 475.0 63.3 y = 1.012x - 0.892 0.991 0.075 0.250 4.81 1.07 7.94
10 All-trans- β-carotene 9.42 (425.0), 452.0, 479.0 30.0 y = 1.016x - 1.176 0.990 0.075 0.250 5.19 1.09 6.36
11 All-trans- ζ-carotene 10.18 380.0, 403.0, 426.0 115.4 y = 1.015x - 1.092 0.991 0.075 0.250 5.69 1.14 7.69
12 All-trans- δ-carotene 11.43 432.0, 457.0, 488.0 71.4 y = 1.014x - 1.015 0.989 0.075 0.250 6.51 1.06 5.22
13 All-trans- γ-carotene 12.09 439.0, 462.0, 492.0 53.3 y = 1.008x - 0.396 0.983 0.075 0.250 6.95 1.02 8.40
14 All-trans-neurosporene 12.40 416.0, 440.0, 469.0 92.3 y = 1.010x - 1.041 0.991 0.075 0.250 7.15 1.25 4.86
15 All-trans-lycopene 15.09 446.0, 472.0, 503.0 73.9 y = 1.027x - 0.630 0.993 0.075 0.250 8.92 1.37 10.67
  1. Parentheses indicate a shoulder.
  2. %III/II represents the ratio of peak heights from the trough between peak II and III.