High-throughput phenotyping of plant resistance to aphids by automated video tracking

Table 3 Comparison of automated video tracking and EPG recording characteristics

	EPG	Video
Plant material	Intact plants	Leaf discs
Min. observation duration	4-8 h	4-8 h
Min. number of replicates (identification rate ±80%)	3-5	15-20
Maximum sample size per set up	± 8	± 100
Preparation time per sample	± 5 min	± 2 min
Annotation of electrical patterns/video images	± 15 min	Automated

Minimum duration of observations and minimum sample sizes were estimated with simulations. Preparation time per replicate reflects a rough estimation of time required for an experienced person to prepare one arena, resp. plant-aphid individual, for a recording.

ISSN: 1746-4811