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Table 1 Feeding behaviour of M. persicae on two Arabidopsis accessions and N. ribisnigri on two lettuce cultivars

From: High-throughput phenotyping of plant resistance to aphids by automated video tracking

  

Arabidopsis – M. persicae

Lettuce – N. ribisnigri

  

Co-2 R

Sanna-2 S

Corbana R

Terlana S

EPG intact plants

Total duration non-penetration

139 ± 15

60.2 ± 8.1 Ts***

121 ± 16

29.2 ± 5.8 Tl***

Total duration phloem feeding

110 ± 13

296 ± 31 M***

2.34 ± 1.68

399 ± 17 M***

Total duration phloem feeding (>10 min)

27.7 ± 7.9

259 ± 32 M***

0.00 ± 0.00

399 ± 17 M***

Total duration other penetration activities

231 ± 15.8

124 ± 28 M**

357 ± 16

51.5 ± 14 T***

Number of non-penetrations

57.7 ± 6.2

22.4 ± 3.1 M***

27.8 ± 7.58

7.58 ± 1.33 T***

Number of short probes (<3 min)

49.8 ± 5.8

18.4 ± 2.4 M***

13.7 ± 2.0

4.53 ± 1.17 M***

Number of phloem feeding events

6.37 ± 0.55

4.28 ± 0.70 T*

0.11 ± 0.07

1.16 ± 0.12 M***

Mean duration of phloem feeding events

17.6 ± 1.4

117 ± 26 M***

22.2 ± 6.16

379 ± 26 M*

Latency to first phloem feeding event

188 ± 25

111 ± 26 M*

228 ± 94

106 ± 17 M

Contribution salivation to phloem phase (%)

13.3 ± 2.2

2.45 ± 1.3 M***

98.7 ± 1.0

1.15 ± 0.3 M***

EPG leaf discs

Total duration non-penetration

164 ± 48

173 ± 59 M

-

-

Total duration phloem feeding

220 ± 42

201 ± 48 T

-

-

Total duration phloem feeding (>10 min)

158 ± 46

177 ± 47 T

-

-

Total duration other penetration activities

95.3 ± 25.7

106 ± 17 T

-

-

Number of non-penetrations

19.1 ± 5.7

14.4 ± 3.0 Ts

-

-

Number of short probes (<3 min)

16.6 ± 4.8

8.63 ± 2.0 Ts

-

-

Number of phloem feeding events

8.11 ± 1.53

2.63 ± 0.84 M*

-

-

Mean duration of phloem feeding events

34.2 ± 10.8

99.2 ± 31.7 Ts*

-

-

Latency to first phloem feeding event

144 ± 51

250 ± 53 T

-

-

Contribution salivation to phloem phase (%)

3.03 ± 0.6

3.63 ± 2.2 M

-

-

Video leaf discs

Total duration non-probing

61.5 ± 9.7

42.6 ± 9.4 T

243 ± 27

148 ± 25 T*

Total duration long probes (>25 min)

338 ± 20

377 ± 17 T

153 ± 26

283 ± 25 T***

Total duration sust. probes (>35 min)

276 ± 27

353 ± 19 M*

132 ± 24

260 ± 25 T***

Total duration other probes (<= 25 min)

80.6 ± 12.4

60.5 ± 9.9 T

84.1 ± 7.0

48.4 ± 9.3 M***

Number of non-penetrations

33.2 ± 3.9

22.1 ± 3.8 T

31.1 ± 3.1

23.0 ± 2.6 T

Number of short probes (<3 min)

20.3 ± 3.1

12.4 ± 3.4 T

22.4 ± 3.1

15.6 ± 2.3 M

Number of long probes (> = 25 min)

5.95 ± 0.36

4.71 ± 0.36 M*

2.19 ± 0.33

3.25 ± 0.30 T*

Mean duration of long probes (> = 25 min)

62.8 ± 7.0

90.0 ± 9.8 M*

72.4 ± 9.5

99.8 ± 11.2 M*

Latency to first long probe (> = 25 min)

18.5 ± 5.8

27.1 ± 7.1 M

142 ± 27

65.1 ± 14.2 M*

Total duration not moving (min)

445 ± 7.3

465 ± 3.5 M

257 ± 27

350 ± 25 T*

Total distance moved (cm)

46.9 ± 4.6

34.0 ± 4.6 T*

204 ± 47

103 ± 23 T

Max velocity (mm/s)

0.72 ± 0.12

0.53 ± 0.05 T

2.78 ± 0.54

1.88 ± 0.20 M

  1. Within each EPG and video tracking experiment it was tested whether aphid behaviour differed between the two plant lines. The mean duration and latency were only calculated if the corresponding events did occur (all samples of M. persicae; N. ribsinigri: EPG n = 2 Corbana and n = 20 Terlana, video tracking n = 21 Corbana and n = 26 Terlana).
  2. Means ± standard error, *P < 0.05; **P < 0.01; ***P < 0.001, T = Student’s t-test, Ts = Student’s t-test on square root transformed data, Tl = Student’s t-test on log transformed data, M = Mann–Whitney U test, R = resistant plant line, S = susceptible plant line, time is represented in min.
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Plant Methods

ISSN: 1746-4811