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Table 1 Feeding behaviour of M. persicae on two Arabidopsis accessions and N. ribisnigri on two lettuce cultivars

From: High-throughput phenotyping of plant resistance to aphids by automated video tracking

   Arabidopsis – M. persicae Lettuce – N. ribisnigri
   Co-2 R Sanna-2 S Corbana R Terlana S
EPG intact plants Total duration non-penetration 139 ± 15 60.2 ± 8.1 Ts*** 121 ± 16 29.2 ± 5.8 Tl***
Total duration phloem feeding 110 ± 13 296 ± 31 M*** 2.34 ± 1.68 399 ± 17 M***
Total duration phloem feeding (>10 min) 27.7 ± 7.9 259 ± 32 M*** 0.00 ± 0.00 399 ± 17 M***
Total duration other penetration activities 231 ± 15.8 124 ± 28 M** 357 ± 16 51.5 ± 14 T***
Number of non-penetrations 57.7 ± 6.2 22.4 ± 3.1 M*** 27.8 ± 7.58 7.58 ± 1.33 T***
Number of short probes (<3 min) 49.8 ± 5.8 18.4 ± 2.4 M*** 13.7 ± 2.0 4.53 ± 1.17 M***
Number of phloem feeding events 6.37 ± 0.55 4.28 ± 0.70 T* 0.11 ± 0.07 1.16 ± 0.12 M***
Mean duration of phloem feeding events 17.6 ± 1.4 117 ± 26 M*** 22.2 ± 6.16 379 ± 26 M*
Latency to first phloem feeding event 188 ± 25 111 ± 26 M* 228 ± 94 106 ± 17 M
Contribution salivation to phloem phase (%) 13.3 ± 2.2 2.45 ± 1.3 M*** 98.7 ± 1.0 1.15 ± 0.3 M***
EPG leaf discs Total duration non-penetration 164 ± 48 173 ± 59 M - -
Total duration phloem feeding 220 ± 42 201 ± 48 T - -
Total duration phloem feeding (>10 min) 158 ± 46 177 ± 47 T - -
Total duration other penetration activities 95.3 ± 25.7 106 ± 17 T - -
Number of non-penetrations 19.1 ± 5.7 14.4 ± 3.0 Ts - -
Number of short probes (<3 min) 16.6 ± 4.8 8.63 ± 2.0 Ts - -
Number of phloem feeding events 8.11 ± 1.53 2.63 ± 0.84 M* - -
Mean duration of phloem feeding events 34.2 ± 10.8 99.2 ± 31.7 Ts* - -
Latency to first phloem feeding event 144 ± 51 250 ± 53 T - -
Contribution salivation to phloem phase (%) 3.03 ± 0.6 3.63 ± 2.2 M - -
Video leaf discs Total duration non-probing 61.5 ± 9.7 42.6 ± 9.4 T 243 ± 27 148 ± 25 T*
Total duration long probes (>25 min) 338 ± 20 377 ± 17 T 153 ± 26 283 ± 25 T***
Total duration sust. probes (>35 min) 276 ± 27 353 ± 19 M* 132 ± 24 260 ± 25 T***
Total duration other probes (<= 25 min) 80.6 ± 12.4 60.5 ± 9.9 T 84.1 ± 7.0 48.4 ± 9.3 M***
Number of non-penetrations 33.2 ± 3.9 22.1 ± 3.8 T 31.1 ± 3.1 23.0 ± 2.6 T
Number of short probes (<3 min) 20.3 ± 3.1 12.4 ± 3.4 T 22.4 ± 3.1 15.6 ± 2.3 M
Number of long probes (> = 25 min) 5.95 ± 0.36 4.71 ± 0.36 M* 2.19 ± 0.33 3.25 ± 0.30 T*
Mean duration of long probes (> = 25 min) 62.8 ± 7.0 90.0 ± 9.8 M* 72.4 ± 9.5 99.8 ± 11.2 M*
Latency to first long probe (> = 25 min) 18.5 ± 5.8 27.1 ± 7.1 M 142 ± 27 65.1 ± 14.2 M*
Total duration not moving (min) 445 ± 7.3 465 ± 3.5 M 257 ± 27 350 ± 25 T*
Total distance moved (cm) 46.9 ± 4.6 34.0 ± 4.6 T* 204 ± 47 103 ± 23 T
Max velocity (mm/s) 0.72 ± 0.12 0.53 ± 0.05 T 2.78 ± 0.54 1.88 ± 0.20 M
  1. Within each EPG and video tracking experiment it was tested whether aphid behaviour differed between the two plant lines. The mean duration and latency were only calculated if the corresponding events did occur (all samples of M. persicae; N. ribsinigri: EPG n = 2 Corbana and n = 20 Terlana, video tracking n = 21 Corbana and n = 26 Terlana).
  2. Means ± standard error, *P < 0.05; **P < 0.01; ***P < 0.001, T = Student’s t-test, Ts = Student’s t-test on square root transformed data, Tl = Student’s t-test on log transformed data, M = Mann–Whitney U test, R = resistant plant line, S = susceptible plant line, time is represented in min.