From: Advances in plant gene-targeted and functional markers: a review
 | Targeted fingerprinting markers (TFMs) | |||||
---|---|---|---|---|---|---|
 | DALP | PAAP | SRAP | TRAP | CoRAP | SCoT |
Abundance | High | High | High | Medium | Medium | High |
Reproducibility | Medium | Low | High | High | Medium | Medium |
Polymorphism | High | Medium | High | High | Medium | Medium |
Prior sequence information | No | No | No | Yes | Yes | No |
Visualization | Silver stained PAGE | Agarose gel electrophoresis | Silver stained PAGE or agarose gel electrophoresis | Silver stained PAGE | Silver stained PAGE | Agarose gel electrophoresis |
Specificity | Low | Low | High | High | Medium | Low |
Size of bands | 200-1,000Â bp | 200-1,200Â bp | 50-1,500Â bp | 50-900Â bp | 50-900Â bp | 200-1,500Â bp |
Homoplasy | Medium | High | Medium | Medium | Not reported | High |
Reaction artifacts | Â | Â | Â | Â | Â | Â |
i. Uniparental bands | May occur | May occur | May occur | No | No | May occur |
ii. Heteroduplexes | Yes | Yes | May occur | May occur | Yes | May occur |
iii. Nested priming | Yes | Yes | Yes | Yes | Yes | Yes |
iv. Other | Complexity of banding patterns may increase with polyploidy | |||||
 | Limited independence of bands | Limited independence of bands | Limited independence of bands | No | Not reported | Limited independence of bands |
 | Not reported | No | Overlooked co-dominancy | Overlooked co-dominancy | Overlooked co-dominancy | Overlooked co-dominancy |