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Table 4 Comparison of various aspects of targeted fingerprinting markers

From: Advances in plant gene-targeted and functional markers: a review

  Targeted fingerprinting markers (TFMs)
  DALP PAAP SRAP TRAP CoRAP SCoT
Abundance High High High Medium Medium High
Reproducibility Medium Low High High Medium Medium
Polymorphism High Medium High High Medium Medium
Prior sequence information No No No Yes Yes No
Visualization Silver stained PAGE Agarose gel electrophoresis Silver stained PAGE or agarose gel electrophoresis Silver stained PAGE Silver stained PAGE Agarose gel electrophoresis
Specificity Low Low High High Medium Low
Size of bands 200-1,000 bp 200-1,200 bp 50-1,500 bp 50-900 bp 50-900 bp 200-1,500 bp
Homoplasy Medium High Medium Medium Not reported High
Reaction artifacts       
i. Uniparental bands May occur May occur May occur No No May occur
ii. Heteroduplexes Yes Yes May occur May occur Yes May occur
iii. Nested priming Yes Yes Yes Yes Yes Yes
iv. Other Complexity of banding patterns may increase with polyploidy
  Limited independence of bands Limited independence of bands Limited independence of bands No Not reported Limited independence of bands
  Not reported No Overlooked co-dominancy Overlooked co-dominancy Overlooked co-dominancy Overlooked co-dominancy