Table 3 Comparison of various aspects of resistance-gene based markers and RNA-based markers
From: Advances in plant gene-targeted and functional markers: a review
| Â | Resistance-gene based markers (RGMs) | RNA-based markers (RBMs) | ||||
|---|---|---|---|---|---|---|
| Â | RGAP | NBS-profiling | iSNAP | cDNA-AFLP | cDNA-RFLP | EST-SSR |
Abundance | High | High | High | High | High | Medium |
Reproducibility | Medium | High | High | High | High | High |
Polymorphism | Medium | High | High | High | Medium | Medium |
Prior sequence information | No | No | Yes | No | No | Yes |
Visualization | Silver stained PAGE | Silver stained PAGE | Silver stained PAGE | Silver stained PAGE | Silver stained PAGE | Silver stained PAGE |
Specificity | High | High | High | Medium | High | High |
Size of bands | 200-1,500Â bp | 70-600Â bp | 100-1,500Â bp | 100-1,000Â bp | 100-3,500Â bp | 100-400Â bp |
Homoplasy | Low | Low | Not reported | Medium | Low | Medium |
Reaction artifacts | Â | Â | Â | Â | Â | Â |
i. Uniparental bands | No | No | Not reported | Rare | No | No |
ii. Heteroduplexes | Not reported | No | May occur | No | No | Yes |
iii. Nested priming | May occur | No | May occur | No | No | No |
iv. Other | Generated bands may evolve under selection | No | No | No | No | |