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Table 3 Comparison of various aspects of resistance-gene based markers and RNA-based markers

From: Advances in plant gene-targeted and functional markers: a review

  Resistance-gene based markers (RGMs) RNA-based markers (RBMs)
  RGAP NBS-profiling iSNAP cDNA-AFLP cDNA-RFLP EST-SSR
Abundance High High High High High Medium
Reproducibility Medium High High High High High
Polymorphism Medium High High High Medium Medium
Prior sequence information No No Yes No No Yes
Visualization Silver stained PAGE Silver stained PAGE Silver stained PAGE Silver stained PAGE Silver stained PAGE Silver stained PAGE
Specificity High High High Medium High High
Size of bands 200-1,500 bp 70-600 bp 100-1,500 bp 100-1,000 bp 100-3,500 bp 100-400 bp
Homoplasy Low Low Not reported Medium Low Medium
Reaction artifacts       
i. Uniparental bands No No Not reported Rare No No
ii. Heteroduplexes Not reported No May occur No No Yes
iii. Nested priming May occur No May occur No No No
iv. Other Generated bands may evolve under selection No No No No