Figure 6

Schematic representation of iPBS. For successful amplification LTR retrotransposons must be in a head-to-head orientation. Primers are designed to anneal to the PBS regions (blue box), in the internal core (grey box), and have flanking regions in CA and TG in the LTR motif (red box). Different amplicons (brown bars) are generated containing the LTRs and the PBS regions plus the intervening genomic segment of variable length.