Figure 4

Data spread analysis of¹⁵N-nitrate influx for individual oocytes injected with water or RNA. Injected oocytes were incubated in MBS solution containing 0.5 mM Na¹⁵NO₃ for 8 and 16 h. Delta ¹⁵N influx of individual oocytes injected with water (blue), RNA prepared from synthetic template DNA (red) and original genes (green) were compared. The data are from 10-15 cells after 8 h (A) or 16 h (B) incubation. The line (orange) represents the expected ¹⁵N influx value for an oocyte injected with the original plant DNA-template RNA. At both 8 h (A) and 16 h (B), 100% of the synthetic-template RNA injected oocytes were above this threshold line, while the equivalent figure for the original plant DNA were 14 and 17% at 8 and 16 h respectively.