Data spread analysis of15N-nitrate influx for individual oocytes injected with water or RNA. Injected oocytes were incubated in MBS solution containing 0.5 mM Na15NO3 for 8 and 16 h. Delta 15N influx of individual oocytes injected with water (blue), RNA prepared from synthetic template DNA (red) and original genes (green) were compared. The data are from 10-15 cells after 8 h (A) or 16 h (B) incubation. The line (orange) represents the expected 15N influx value for an oocyte injected with the original plant DNA-template RNA. At both 8 h (A) and 16 h (B), 100% of the synthetic-template RNA injected oocytes were above this threshold line, while the equivalent figure for the original plant DNA were 14 and 17% at 8 and 16 h respectively.