Figure 4

Visualization of H3K4me3 and H3K9me2 in specific cells in different plants. Immunodetection was carried out with specific antibodies against H3K4me3 and H3K9me2. Confocal images of DAPI, H3K4me3, H3K9me2 and merged signals are shown from left to right and represent specific zones magnified from dashed squares shown in Figure 3, to verify that the signal in section tissues is free of background noise and it is inside cells. (A) Meristematic cells of globular embryo in Coffea canephora. (B) Callus cells of Arabidopsis thaliana. (C) Shoot apex cells of Agave tequilana. (D) Epidermic layer (yellow arrowhead) and endothecium cells (white arrowhead) of lobes anthers in Capsicum chinense. (E) Epidermis and collenchyme cells of Cedrela odorata. In all species, the H3K4me3 was observed to be particularly abundant in the biggest and largest cells; whereas the H3K9me2 was visualized in the nuclei of cells preferentially as flourescent spots.