Figure 3

Immunodetection of histone modifications in tissues of several plant species. Immunodetection was performed using antibodies against H3K4me3 and H3K9me2, using the histone H3 as positive control. Panels from left to right show the confocal images of transmitted light, DAPI, histone H3, H3K4me3 and H3K9me2. Dashed squares represent the close-up of the sites detected by immunoflourescence with the antibodies mentioned above in the globular embryo of Coffea canephora (A), in meristematic zones in the callus of Arabidopsis thaliana (B), in the shoot apex of Agave tequilana (C), in the endothecium (yellow arrowhead) and in the lobes cells of anthers (white arrowhead) in Capsicum chinense (D) and in the proximal cells to epidermis of Cedrela odorata (E).