Figure 2
From: High-level diterpene production by transient expression in Nicotiana benthamiana
Production of casbene and of levopimaradiene. (A) Total ion chromatograms of hexane washes from N. benthamiana leaves agro-infiltrated with p19 and either the empty T-DNA vector (control) or RcCS or GbLS. Peaks for casbene, levopimaradiene and other novel products (a – e) are indicated. (B) Casbene and levopimaradiene content in hexane washes from leaves agro-infiltrated with p19 and RcCS or GbLS, respectively. Samples were taken five days post-infiltration from two individual N. benthamiana plants, each of them having two completely infiltrated leaves. Mean casbene and levopimaradiene values ± SEM are presented (n = 16, two independent experiments). Sclareol was used as internal standard (ISTD) for quantification.