A device for single leaf labelling with CO2 isotopes to study carbon allocation and partitioning in Arabidopsis thaliana

Table 1 Time course of carbon partitioning into the major carbon compound classes in the labelled leaf

			% of ¹⁴C recovered in each fraction related to the total amount per tissue
			After pulse	15 min chase	30 min chase	60 min chase	180 min chase
Labeled leaf ¹			100	100	100	100	100
	Soluble		77.0 ± 2.2	58.4 ± 3.5	59.3 ± 2.1	46.2 ± 3.4	44.7 ± 2.4
		Neutral	14.6 ± 0.9	23.1 ± 1.2	22.8 ± 1.9	11.6 ± 2.0	7.3 ± 0.9
		Acidic	27.7 ± 0.9	15.1 ± 0.7	17.0 ± 1.8	20.0 ± 1.5	25.9 ± 1.8
		Basic	24.9 ± 1.4	14.3 ± 1.2	11.3 ± 1.3	8.2 ± 0.5	5.4 ± 0.5
	Insoluble		17.3 ± 2.0	30.5 ± 4.3	36.6 ± 1.9	42.5 ± 4.6	45.1 ± 4.7
		Starch	16.6 ± 1.1	27.7 ± 2.4	29.3 ± 2.2	34.4 ± 3.1	37.2 ± 3.2
		Protein	0.8 ± 0.2	3.1 ± 0.7	3.1 ± 0.5	4.6 ± 0.5	4.2 ± 0.5
		Others	0.3 ± 0.1	2.0 ± 0.6	2.3 ± 0.3	3.8 ± 0.2	3.7 ± 0.4
	EtOH soluble		1.7 ± 0.1	4.0 ± 0.6	4.1 ± 0.4	5.6 ± 0.2	5.7 ± 0.3

¹Leaf 8 of Col-0 plants was labelled for 5 min with ¹⁴CO₂, 6 h into the light, followed by a chase period of 0, 15, 30, 60 or 180 min. After the chase, the labelled leaf was harvested and the incorporation of ¹⁴C was determined. The sum of the label in the labelled leaf is set to 100%. The relative amount of ¹⁴C incorporated into the different compound classes (as a percentage of the total label in the leaf) is shown. Mean ± SE (n ≥ 5).

ISSN: 1746-4811