Table 2 Summary of TILLING primers tested, quality of PCR products, CJE digestion products and polymorphisms detected
From: Validation of doubled haploid plants by enzymatic mismatch cleavage
Primer pair | PCR product in the parental lines | CJE digestion | Polymorphisms detected between parental lines | Tested in DH material | ||
|---|---|---|---|---|---|---|
|  | GP1 | HOR1606 | GP2 | HOR 1606 | GP + HOR1606 |  |
rdg2a_F1 + R1 | N | Y | --- | --- | --- | N |
rdg2a_F2 + R2 | N | Y | --- | --- | --- | N |
rdg2a_F3 + R3 | N | W | --- | --- | --- | N |
nbs2-rdg2a_F1 + R1 | W | W | ? | ? | N | N |
nbs2-rdg2a_F2 + R2 | Y | Y | F | C | Y | Y |
nbs2-rdg2a_F3 + R3 | W | W | ? | ? | N | N |
nbs3-rdg2a_F1 + R1 | Y | Y | F | C | Y | Y |
nbs3-rdg2a_F2 + R2 | N | N | --- | --- | --- | N |
HVgna1f + r | N | N | --- | --- | --- | N |
HVraa1f + R | W | W | ? | ? | N | N |
HV_hpa1F + R | W | W | ? | ? | N | N |
HV_Mlo9-F1 + R1 | Y | Y | F | F | Y | Y |
HV_Mlo9-F2 + R2 | Y | Y | F | F | Y | Y |
HvHox1-F1 + R1 | Y | Y | F | F | N | N |
HV_Kap1_c1_f + r | Y | Y | F | F | Y | Y |
HV_Kap1_d1_f + r | Y | Y | F | F | Y | Y |
HV_Kap1_e1_f + r | Y | Y | F | F | Y | Y |
HV_Kap1_g1_f + r | Y | Y | F | F | N | N |
HV_Kap1_h1_f + r | Y | Y | F | F | N | N |
HV_Kap1_i1_f + r | Y | Y | F | F | N | N |
Hv_mloA-F + R | --- | --- | --- | --- | Y | Y |
Hv_mloB-F + R | --- | --- | --- | --- | Y | Y |
Hv_mloC-F + R | --- | --- | --- | --- | Y | Y |
Hv_mloD-F + R | --- | --- | --- | --- | Y | Y |
Hv_mloE-F + R | --- | --- | --- | --- | N | N |
Hv_Mla1-3 F + 3R | --- | --- | --- | --- | N | N |