Figure 2
From: Validation of doubled haploid plants by enzymatic mismatch cleavage
Doubled haploid screening and polymorphism discovery in the nbs2-rdg2a locus. A 1500Â bp gene fragment of the barley nbs2-rdg2a locus was PCR amplified and subjected to enzymatic mismatch cleavage. Parental line Golden Promise is homozygous in the gene region while HOR1606 shows banding indicative of co-amplification of homologous gene copies (lanes 1 and 2 respectively). Heterozygous polymorphisms marked by arrows are found in a synthetic mixture of parental genotypes and in DNA from an F1 hybrid of the cross of the two parents (lanes 3 & 4). Doubled haploids in lanes 5-13 are all homozygous. Banding patterns indicate if the gene target originates from GP or HOR1606.