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Figure 7 | Plant Methods

Figure 7

From: Cell surface and cell outline imaging in plant tissues using the backscattered electron detector in a variable pressure scanning electron microscope

Figure 7

BSE imaging of epidermal cell surface features and internal organelles in a critical point dried A. thaliana seed (A,B), cotton flower petal (C,D), cotton leaf (E,F) and rice leaf (G, H). The same areas of tissue were imaged using 10 kV (A,C,E,G) or 20 kV (B, D, F, H) accelerating voltages (10 Pa chamber pressure). Arrowheads in (B) and (D) indicate cell outlines, in (E) indicate wax deposits on leaf surface, and in (G) and (H) silica deposits on the leaf surface. n = nucleus; s = starch granules. Scale bars = 20 μm (A–D; bar shown in D), 30 μm (EH; bar shown in H).

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