BSE and SE imaging of hydrated A. thaliana rosette leaves. The same area of leaf was imaged frozen on a Peltier-cooled stage with the BSE (A) or SE (B) detectors in VP mode (Accelerating voltage 20 kV and chamber pressure 10 Pa). Different leaves were imaged in extended pressure mode with water vapour as the imaging gas with the BSE (C) and SE (D) detectors. EP-SEM conditions were 82% humidity, 600–700 Pa chamber pressure, 2-3°C Peltier stage temperature, and 20–25 kV accelerating voltage. Scale bar = 40 μm (A–D, bar shown in D).