Reducing charging for imaging surface details and cell wall outlines in critical point dried A. thaliana rosette leaves under VP mode. Uncoated (A) and carbon-coated (B) leaf imaged at 10 kV accelerating voltage with the VP-SE detector for surface topography. (C-F) BSE (C, E) and SE (D, F) images were collected at 20 kV from the same area of leaf, either coated with carbon (C, D), or gold (E, F), respectively. Chamber pressure 10 Pa. Scale bar = 60 μm (A–F, bar shown in F).