Skip to main content
Figure 4 | Plant Methods

Figure 4

From: Cell surface and cell outline imaging in plant tissues using the backscattered electron detector in a variable pressure scanning electron microscope

Figure 4

Reducing charging for imaging surface details and cell wall outlines in critical point dried A. thaliana rosette leaves under VP mode. Uncoated (A) and carbon-coated (B) leaf imaged at 10 kV accelerating voltage with the VP-SE detector for surface topography. (C-F) BSE (C, E) and SE (D, F) images were collected at 20 kV from the same area of leaf, either coated with carbon (C, D), or gold (E, F), respectively. Chamber pressure 10 Pa. Scale bar = 60 μm (A–F, bar shown in F).

Back to article page

Plant Methods

ISSN: 1746-4811