Effect of chamber gas pressure on imaging of cell wall outlines in critical point dried A. thaliana rosette leaf. BSE (A,C,E,G) and SE (B,D,F,H) images were collected from the same area of a single leaf, at 10 (A, B), 50 (C, D), 100 (E, F), and 200 Pa (G, H) chamber pressure, respectively. Brightness and contrast levels were not changed for BSE images. Accelerating voltage 20 kV. Scale bar = 60 μm (A–H, bar shown in H).