Imaging of epidermal cells with widefield and confocal microscopy. Cleared A. thaliana rosette leaves imaged by differential interference contrast (DIC) widefield microscopy (A, B). Fresh A. thaliana leaves imaged by CLSM using GFP targeted to the plasma membrane (C, D). CLSM imaging of propidium iodide stained fresh A. thaliana cotyledon (E). Autofluorescence of a fresh barley leaf detected after UV excitation in CLSM (F). Arrowheads indicate cell wall outlines. Scale bars = 30 μm (D), 50 μm (B, E), and 100 μm (A, C, F).