Simplified Arabidopsis hydroponics growth method. Flow chart outlining the timeline and key steps in the process. Timing (in bold) on right of arrows indicate time between steps (d: days). Images on right-hand panel showing setup of seed germination and representative images of seedlings and mature plants, including view of roots contained within centrifuge tubes of 5-week old plant. Also refer to protocol, and Additional file 2 and online tutorial video (http://www.youtube.com/watch?v=c9neVLaS63c) for more detailed descriptions of the equipment set-up.