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Table 2 Primers used in this study
| Primer name | Sequence 5′ to 3′ |
|---|---|
| PDS_gRNA1_BsaIf | TGTGGTCTCAATTGCCGTTAATTTGAGAGTCCAGTTTTAGAGCTAGAAATAGCAAG |
| PDS_gRNA2_BsaIf | TGTGGTCTCAATTGTCAAGATGTTTGCTTGCAAGTTTTAGAGCTAGAAATAGCAAG |
| gRNA_AGCG_BsaIr | TGTGGTCTCAAGCGTAATGCCAACTTTGTAC |
| Cas9_1f | GAGGAAGACAAAATGGACAAGAAGTACTCCATTGGG |
| Cas9_1r | GAGGAAGACAAAGTCTCTTCTGATTTGCGAGTCATCCA |
| Cas9_2f | GAGGAAGACAAGACTATCACTCCCTGGAACTTCGAG |
| Cas9_2r | GAGGAAGACAAATCTTCTTTGAGCTGTTTCACGGTAACT |
| Cas9_3f | GAGGAAGACAAAGATTATTTCAAAAAGATTGAATG |
| Cas9_3r | GAGGAAGACAAGAGACTGTCCCCCTGGCCAGAAACTTG |
| Cas9_4f | GAGGAAGACAATCTCCACGAGCACATCGCTAATCTTGCAGG |
| Cas9_4r | GAGGAAGACAAGAAACCAGCTTAGACTTCAGAGTAATA |
| Cas9_5f | GAGGAAGACAATTTCAGATTTCAGAAAGGACTTTCAG |
| Cas9_5r | GAGGAAGACAAAGATCCTTTGAGCTTTTCATAGTGGCTGG |
| Cas9_6f | GAGGAAGACAAATCTCCCGAAGATAATGAGCAGAAG |
| Cas9_6-1r | GAGGAAGACAAAAGCTCACACCTTCCTCTTCTTCTTGG |
| PDS_MlyIF | GCTTTGCTTGAGAAAAGCTCTC |
| PDSseqr5 | TTTAAAGGATTAAAGTCCTTTGTCA |
| M13 forward | GTTGTAAAACGACGGCCAGT |
| M13 reverse | CAGGAAACAGCTATGACC |
