Table 2 Primers used in this study
Primer name | Sequence 5′ to 3′ |
|---|---|
PDS_gRNA1_BsaIf | TGTGGTCTCAATTGCCGTTAATTTGAGAGTCCAGTTTTAGAGCTAGAAATAGCAAG |
PDS_gRNA2_BsaIf | TGTGGTCTCAATTGTCAAGATGTTTGCTTGCAAGTTTTAGAGCTAGAAATAGCAAG |
gRNA_AGCG_BsaIr | TGTGGTCTCAAGCGTAATGCCAACTTTGTAC |
Cas9_1f | GAGGAAGACAAAATGGACAAGAAGTACTCCATTGGG |
Cas9_1r | GAGGAAGACAAAGTCTCTTCTGATTTGCGAGTCATCCA |
Cas9_2f | GAGGAAGACAAGACTATCACTCCCTGGAACTTCGAG |
Cas9_2r | GAGGAAGACAAATCTTCTTTGAGCTGTTTCACGGTAACT |
Cas9_3f | GAGGAAGACAAAGATTATTTCAAAAAGATTGAATG |
Cas9_3r | GAGGAAGACAAGAGACTGTCCCCCTGGCCAGAAACTTG |
Cas9_4f | GAGGAAGACAATCTCCACGAGCACATCGCTAATCTTGCAGG |
Cas9_4r | GAGGAAGACAAGAAACCAGCTTAGACTTCAGAGTAATA |
Cas9_5f | GAGGAAGACAATTTCAGATTTCAGAAAGGACTTTCAG |
Cas9_5r | GAGGAAGACAAAGATCCTTTGAGCTTTTCATAGTGGCTGG |
Cas9_6f | GAGGAAGACAAATCTCCCGAAGATAATGAGCAGAAG |
Cas9_6-1r | GAGGAAGACAAAAGCTCACACCTTCCTCTTCTTCTTGG |
PDS_MlyIF | GCTTTGCTTGAGAAAAGCTCTC |
PDSseqr5 | TTTAAAGGATTAAAGTCCTTTGTCA |
M13 forward | GTTGTAAAACGACGGCCAGT |
M13 reverse | CAGGAAACAGCTATGACC |