Table 3 Profiling primer sequences and annealing temperatures
From: Novel applications of motif-directed profiling to identify disease resistance genes in plants
Primer name | Primer sequence | Ta | Reference |
|---|---|---|---|
Pk1Fa | GGyTTTGGwrinGTyTACAAAGG | 52 | [42]/this study |
PK1Fb | GGyTTTGGwrinGTyTAtAAGGG | 52 | [42]/this study |
PK4R1a | yykrGCyAGyCCAAAATC | 54 | [42]/this study |
PK3Fb | rTyGTrCATAGrGACATCAA | 54 | [42]/this study |
NBS1 | GCiArwGTwGTyTTiCCyrAiCC | 55 | [49] |
NBS2 | GTwGTyTTiCCyrAiCCissCAT | 60 | [24] |
NBS5a6 | yyTkrThGTmiTkGATGAyrTiTGG | 55 | [49] |
NBS9 | TGTGGAGGrTTACCTCTAGC | 55 | [24] |
chcR1 | TGGmCkrAGAAAmCCTTCwGCCATC | 66 | This study |
chcR2 | CCwArrCCwsCCATwCCyACTAT | 62 | This study |
chcF2 | CTACCAmkyCGAsArAcAGATTCC | 64 | This study |
NBS13R | AAGAArCATGCdATATCTArAAATAT | 55 | [30] |
NBS15F | ATGCATGAyTTrATwvAAGAbATGGG | 55 | [30] |
TIR300Fc | TAGTrAAGATyATGGAATGCA | 55 | This study |
TIR300F | nTAGTrAAGAyATGGAATGC | 55 | [30] |
TIR9256 | ATGGCATCTTCTTCTTCTTCTTTTGCG | 55 | [30] |
TIR3R | CTTCACTAnTTCATyCAAGCACC | 55 | [30] |
TIRWCF | AGAATTAyrCiACrTCiAGrTGGTG | 60 | This study |
Adapt-upper | ACTCGATTCTCAACCCGAAAGTATAGATCCCA | Â | [24] |
Adapt-lower blunt | TGGGATCTATACTT-NH2 | Â | This study |
TG233F | CATGCCTTTTTCTTGGGATG | Â | This study |
TG233R | TGGAACCCCTTTAACTGTGC | Â | This study |
198E12T-F | GACTCTGCCGTGATTGCTGAA | Â | This study |
198E12T-R | CACCGGGAAGACGCTGTTT | Â | This study |
Aseq primer | GCCTCCCTCGCGCCATCAG | Â | Roche |
Bseq primer | GCCTTGCCAGCCCGCTCAG | Â | Roche |
454Adpt Primer | GCCTCCCTCGCGCCATCAGGTTTACTCGATTCTCAACCCGAAAG | Â | This study |
454B01-NBS5a | GCCTTGCCAGCCCGCTCAGACGAGTGCGT yyTkrThGTmiTkGATGAYGTiTGG | Â | This study |
454B02-NBS5a | GCCTTGCCAGCCCGCTCAGACGCTCGACA yyTkrThGTmiTkGATGAYGTiTGG | Â | This study |
454B03-NBS5a | GCCTTGCCAGCCCGCTCAGAGACGCACTC yyTkrThGTmiTkGATGAYGTiTGG | Â | This study |
454B04-NBS5a | GCCTTGCCAGCCCGCTCAGAGCACTGTAG yyTkrThGTmiTkGATGAYGTiTGG | Â | This study |
454B05-NBS5a | GCCTTGCCAGCCCGCTCAGATCAGACACG yyTkrThGTmiTkGATGAYGTiTGG | Â | This study |
454B06-NBS5a | GCCTTGCCAGCCCGCTCAGATATCGCGAG yyTkrThGTmiTkGATGAYGTiTGG | Â | This study |
454B07-NBS5a | GCCTTGCCAGCCCGCTCAGCGTGTCTCTA yyTkrThGTmiTkGATGAYGTiTGG | Â | This study |
454B08-NBS5a | GCCTTGCCAGCCCGCTCAGCTCGCGTGTC yyTkrThGTmiTkGATGAYGTiTGG | Â | This study |
454B09-NBS5a | GCCTTGCCAGCCCGCTCAGTAGTATCAGC yyTkrThGTmiTkGATGAYGTiTGG | Â | This study |
454B10-NBS5a | GCCTTGCCAGCCCGCTCAGTCTCTATGCG yyTkrThGTmiTkGATGAYGTiTGG | Â | This study |
454B11-NBS5a | GCCTTGCCAGCCCGCTCAGTGATACGTCT yyTkrThGTmiTkGATGAYGTiTGG | Â | This study |
454B12-NBS5a | GCCTTGCCAGCCCGCTCAGTACTGAGCTA yyTkrThGTmiTkGATGAYGTiTGG | Â | This study |