Novel applications of motif-directed profiling to identify disease resistance genes in plants

Table 1 **Number and sequence validation of markers generated in the SH*RH population using the different profiling primers and restriction enzyme combinations**

Primer	*Alu* I	*Hae* III	*Mse* I	*Rsa* I	*Taq* I	Markers (#)	Tagged bin ranges (#)	Sequenced markers (#)	on target* sequences (#)	on target**
Primer	*Alu* I	*Hae* III	*Mse* I	*Rsa* I	*Taq* I	Markers (#)	Tagged bin ranges (#)	Sequenced markers (#)	on target* sequences (#)	bin ranges (#)
NBS1	24	40	22	50	47	183	34	37	19	8
NBS2	51	36	56	43	50	236	29	37	14	6
NBS5a6	22	33	38	35	34	162	38	82	62	14
NBS9	22	25	18	40	46	151	29	53	13	6
total NBS	119	134	134	168	177	732	56	209	108	23
NBS13R	9	7	7	22	10	55	9	34	27	5
NBS15F	7	15	15	34	20	91	11	33	27	4
TIR300F	23	16	27	27	18	111	17	65	41	6
TIR300Fc	5	26	14	18	nd	63	9	40	32	4
TIR3R	16	35	11	12	15	89	12	38	20	3
TIR9256	13	13	29	23	13	91	13	49	22	2
TIRWCF	8	17	28	47	nd	100	17	49	26	6
total N-like	81	129	131	183	76	600	38	308	195	12
chcF2	14	3	9	4	nd	30	5	5	3	0
chcR1	5	1	12	9	nd	27	5	9	6	2
chcR2	8	5	9	15	nd	37	6	16	14	3
total chc1-like	27	9	30	28	0	94	9	30	23	3
PK1Fa	11	9	6	16	nd	42	12	20	13	4
PK1Fb	14	10	16	9	nd	49	16	20	11	8
PK3Fb	16	14	19	26	nd	75	16	42	11	6
PK4R1a	6	12	12	10	nd	40	13	16	4	2
total PK	47	45	53	61	0	206	35	98	39	19
total	274	317	348	440	253	1632	69	645	365	43

* Confirmed as on target when the sequence had significant homology to known R genes or RGAs.
** Confirmed as on target if the tagged bin range contained at least one on target sequenced marker.
# = numbers of.

ISSN: 1746-4811