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Table 1 Number and sequence validation of markers generated in the SH*RH population using the different profiling primers and restriction enzyme combinations

From: Novel applications of motif-directed profiling to identify disease resistance genes in plants

Primer Alu I Hae III Mse I Rsa I Taq I Markers (#) Tagged bin ranges (#) Sequenced markers (#) on target* sequences (#) on target**
bin ranges (#)
NBS1 24 40 22 50 47 183 34 37 19 8
NBS2 51 36 56 43 50 236 29 37 14 6
NBS5a6 22 33 38 35 34 162 38 82 62 14
NBS9 22 25 18 40 46 151 29 53 13 6
total NBS 119 134 134 168 177 732 56 209 108 23
NBS13R 9 7 7 22 10 55 9 34 27 5
NBS15F 7 15 15 34 20 91 11 33 27 4
TIR300F 23 16 27 27 18 111 17 65 41 6
TIR300Fc 5 26 14 18 nd 63 9 40 32 4
TIR3R 16 35 11 12 15 89 12 38 20 3
TIR9256 13 13 29 23 13 91 13 49 22 2
TIRWCF 8 17 28 47 nd 100 17 49 26 6
total N-like 81 129 131 183 76 600 38 308 195 12
chcF2 14 3 9 4 nd 30 5 5 3 0
chcR1 5 1 12 9 nd 27 5 9 6 2
chcR2 8 5 9 15 nd 37 6 16 14 3
total chc1-like 27 9 30 28 0 94 9 30 23 3
PK1Fa 11 9 6 16 nd 42 12 20 13 4
PK1Fb 14 10 16 9 nd 49 16 20 11 8
PK3Fb 16 14 19 26 nd 75 16 42 11 6
PK4R1a 6 12 12 10 nd 40 13 16 4 2
total PK 47 45 53 61 0 206 35 98 39 19
total 274 317 348 440 253 1632 69 645 365 43
  1. * Confirmed as on target when the sequence had significant homology to known R genes or RGAs.
  2. ** Confirmed as on target if the tagged bin range contained at least one on target sequenced marker.
  3. # = numbers of.