Figure 2

Newbler assembly statistics of 454 reads for different minimum overlap identity parameters. Three orthologous assemblies were performed using 97% minimum alignment identity (mi), 99% and 100%. For each assembly, the number of 454 remaining singletons (no significant overlap to any other 454 read), the number of assembled 454 reads and the number of excluded 454 reads (ultra-short reads, outlier reads, repeat reads) were counted.