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Figure 4 | Plant Methods

Figure 4

From: A valid strategy for precise identifications of transcription factor binding sites in combinatorial regulation using bioinformatic and experimental approaches

Figure 4

EMSA results on previous MYB-binding fragments. (A) Sequences of the probes (5’ → 3’) designed from previous reports. Each pair consisted of one probe based on a reported promoter segment and the other mutated at sites in bold. All predicted cis elements from this study were underlined, in comparison to the previously identified cis region (shaded and in italic). The first probe hosted duplicated cis fragments previously for apple MYB10. (B) EMSA results between the probes of (A) and two expressed MYB1 proteins. The binding patterns of IpMYB1 and MlMYB1 to these probes were shown on two gels (all 12% SDS-PAGE). The DNA marker showed the DNA staining (the upper panel) and the ruler (cm) on the same gel measured the protein staining (the lower panel).

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Plant Methods

ISSN: 1746-4811