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Table 5 Advantages and disadvantages of germination determination methods

From: A high-throughput seed germination assay for root parasitic plants

 

Classical microscope assay

Spectrophotometric MTT assay

Duration of the experiment

7 days

6 days

Duration of the reading step (96 well plate)

2-3 h

< 5 min

Sensitivity to false negative

yes

no

Radicle elongation determination

yes

no