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Figure 3 | Plant Methods

Figure 3

From: A high-throughput seed germination assay for root parasitic plants

Figure 3

pH impact on seed germination and radicle growth in Phelipanche ramosa. Conditionned seeds were stimulated with GR24 (3 nmol L-1) then incubated 7 days in Na, K phosphate medium (1 mmol L-1) at pH 5 (a), 6 (b), 7 (c) and 8 (d). A- photographs were taken after MTT incubation for 6 hours. Scale bars 200 μm. B- pH impact on MTT reduction by absorbance measurement at 530 nm minus at absorbance at 630 nm (± confidence intervals α = 0.05, n = 3), radicle length (± confidence intervals α = 0.05, n = 400), germination percentage were 95% ±4%.

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