Genetic transformation of western clover (Trifolium occidentale D. E. Coombe.) as a model for functional genomics and transgene introgression in clonal pasture legume species

Table 2 Segregation of the bar selection marker in T₁ progeny of T. occidentale translines

Plant line	Total no. seedlings	Observed		Expected		Chi-square	P value
		PCR+	PCR-	PCR+	PCR-
AAT6501	53	44	9	40	13	1.63	0.202
AAT6502	58	35	23	44	14	7.63	0.006
AAT6503	56	43	13	42	14	0.10	0.758
AAT6505	76	59	17	75	1	19	2.3×10^-58

Presence of the bar gene was determined by PCR assay of genomic DNA isolated from leaf tissues of T₁ seedlings. For AAT6501, AAT6502 and AAT6503 the expected ratio is based upon a 3:1 segregation of a single copy T-DNA at a single insertion locus and a 63:1 segregation for AAT6505 of three T-DNA’s at separate insertion loci.

ISSN: 1746-4811