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Table 2 Segregation of the bar selection marker in T 1 progeny of T. occidentale translines

From: Genetic transformation of western clover (Trifolium occidentale D. E. Coombe.) as a model for functional genomics and transgene introgression in clonal pasture legume species

Plant line Total no. seedlings Observed Expected Chi-square P value
   PCR+ PCR- PCR+ PCR-   
AAT6501 53 44 9 40 13 1.63 0.202
AAT6502 58 35 23 44 14 7.63 0.006
AAT6503 56 43 13 42 14 0.10 0.758
AAT6505 76 59 17 75 1 19 2.3×10-58
  1. Presence of the bar gene was determined by PCR assay of genomic DNA isolated from leaf tissues of T1 seedlings. For AAT6501, AAT6502 and AAT6503 the expected ratio is based upon a 3:1 segregation of a single copy T-DNA at a single insertion locus and a 63:1 segregation for AAT6505 of three T-DNA’s at separate insertion loci.