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Figure 1 | Plant Methods

Figure 1

From: Genetic transformation of western clover (Trifolium occidentale D. E. Coombe.) as a model for functional genomics and transgene introgression in clonal pasture legume species

Figure 1

Western clover ( T. occidentale ), shoot regeneration and transformation. (a) white clover (T. repens); compared to (b) T. occidentale (c) prolific regeneration of shoots from an AZ4270 cotyledon following three weeks culture on 1C; (d) shoot regeneration under ammonium glufosinate selection following Agrobacterium-mediated transformation of cotyledon explants; (e) transient GUS expression on cotyledonary explants three days after inoculation with Agrobacterium broth; (f) GUS activity in a trifoliate leaf excised from stably transformed plants approximately eight weeks after transformation.

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Plant Methods

ISSN: 1746-4811