Figure 2
From: Wax ester profiling of seed oil by nano-electrospray ionization tandem mass spectrometry
Thin layer chromatographic separation of seed lipid extracts. Separation of seed lipid extracts of wild type plants (*marked with asterisk) or wax ester accumulating transgenic plants. The silica TLC plate was developed with hexane:diethyl ether:acetic acid (80:20:0.1, v/v/v). Bands for the triacylglycerols (TAG), wax esters (WE) and steryl esters (SE) were identified by analysis of respective lipid class standards. Lipids were visualized by charring after immersion in cupric sulfate solution.