Figure 1From: A simple in vitro method to measure autophosphorylation of protein kinasesDetection of recombinant HAE in E. coli cell lysates by Pro-Q Diamond phosphoprotein stain. Left: Total protein stain showing gel shifted MBP-HAE run alongside MBP-HAE-K711E and MBP parental vector negative control. Right: phosphoprotein stain showing heavy autophosphorylation of MBP-HAE and near complete absence of signal in MBP-HAE-K711E and MBP parental vector lanes. Arrows point to bands identified by lane labels.Back to article page