Quantification of the proportion of diseased tissues caused by Xff CFBP4834-R on five cultivars of bean. Bean leaflets of cultivars Flavert, Michelet, Pike, Wonder and Caprice were inoculated with Xff CFBP4834-R (1.106 CFU ml_1) or mock. Observations were made at 7 (A), and 11 dai (B). On each leaflet diseased area was segmented using the probability-based thresholds. Means of percentages of diseased tissues and standard error of the mean were calculated from two repeats with 4 (7 dai) and 7 (11 dai) leaflets. Treatments denoted by different letters are significantly different (p-value < 0.01) based on the Mann–Whitney test. Asterisks mark significant differences between the mock-inoculated and CFBP4834-R-inoculated leaflets (p-value < 0.01) based on the Mann–Whitney test. Clusters representing the various stages of the alteration of plant tissues were determined by a clustering approach using MCLUST  on the diseased area only.