An efficient flat-surface collar-free grafting method for Arabidopsis thaliana seedlings

Table 1 Effect of growth conditions and cotyledon removal in grafting success

Day length	Sugar	Age (days)	Cotyledon number	Event	Total grafted	Successful	Success %	Observations
SD*	0.5%*	4	0	1	45	23	51
		6*	0*	1	34	31	91
				2	40	31	78
				3	8	8	100
				4	66	56	85
				5	82	61	74
				6	42	39	93
				7	40	23	58	horizontal
			1	1	54	31	57
			2	1	12	5	42
				2	34	7	21
				3	54	14	26
				4	20	7	35
				5	70	31	44
				6	25	13	52
				7	26	11	42
		7	0	1	42	34	81
		8	0	1	35	24	69
	0%	6	0	1	30	9	30	no leaves
			1	1	42	16	38
			1	2	31	9	29	horizontal
LD	0.5%	4	0	1	44	17	39
		4	0	2	33	22	67
		5	0	1	39	20	51
		6	0	1	39	11	28
	0%	5	0	1	38	25	66
				2	25	9	36
				3	23	7	30	no leaves

Seedlings were grown in different day length and sugar conditions and grafted at different ages with two, one or zero cotyledons, as indicated in the table rows. The total number of Arabidopsis plants grafted and the number and percentage of successful grafts out of them is presented. “Event” numbers indicate independent grafting sessions. In “Observations¨, “no leaves” means that no leaves were visible 14 days after grafting, “horizontal” means that the seedlings were grown in a plate placed horizontally in the growth chamber. Asterisks indicate the combination of conditions that produced the highest grafting efficiency. SD, Short days; LD, Long days.

ISSN: 1746-4811