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Table 2 Characterization of different FP variants

From: Emission spectra profiling of fluorescent proteins in living plant cells

Fluorescent protein Excitation maximum (nm) Excitation wave length (nm) Emission maximum according to references (nm) Emission maximum measured in this study (nm) Standard deviation (SD) Number of nuclei analyzed (n) References
eGFP 488 488 509.5 506 1.21 20 [25]
eYFP 514 488 527 522 1 4 [25]
Venus 515 488 528 524 1.04 14 [18]
eCFP 434 405 475; 501 474; 500 1.82; 3.03 16 [25]
Cerulean 433 405 475; 501 474; 496 0.57; 2.67 12 [21]
mCherry 587 559 610 598 1.26 16 [19]
mRFP 584 559 and 561** 608 598 and 596* and 607** 1.17 and 2.19* and 1.13** 33 and 22* and 13** [26]
TagRFP 555 515 583 572 1.56 9 [20]
  1. * indicates experiments performed with free mRFP.
  2. ** indicates experiments performed with Zeiss LSM 710.